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. 1978 May 1;171(2):321-7.
doi: 10.1042/bj1710321.

Iron-binding fragments from the N-terminal and C-terminal regions of human lactoferrin

J M Bluard-Deconinck, J Williams, R W Evans, J van Snick, P A Osinski, P L Masson

Iron-binding fragments from the N-terminal and C-terminal regions of human lactoferrin

J M Bluard-Deconinck et al. Biochem J. .

Abstract

Digestion of lactoferrin with pepsin at pH3.0 gave an iron-binding half-molecule that represents the C-terminal part of the native protein. Tryptic or chymotryptic digestion of 30%-iron-saturated lactoferrin yielded the N- and C-terminal half molecules, which could be separated by DEAE-Sephadex chromatography. The N- and C-terminal fragments did not show any immunological cross-reaction. The carbohydrate of lactoferrin was distributed equally between the two fragments.

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