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. 1983 Apr;80(7):1877-81.
doi: 10.1073/pnas.80.7.1877.

Modulation of the kinetics of cholesterol side-chain cleavage by an activator and by an inhibitor isolated from the cytosol of the cortex of bovine adrenals

P A Warne, N J Greenfield, S Lieberman

Modulation of the kinetics of cholesterol side-chain cleavage by an activator and by an inhibitor isolated from the cytosol of the cortex of bovine adrenals

P A Warne et al. Proc Natl Acad Sci U S A. 1983 Apr.

Abstract

Two modulators of sterol side-chain cleavage activity have been detected in the cytosol from the cortex of bovine adrenals. One is an inhibitor of side-chain cleavage which increases the Km of a purified and reconstituted mitochondrial side-chain cleavage system for both cholesterol and cholesterol sulfate. It also lowers the Vmax of cleavage when cholesterol sulfate is the substrate. The other modulator is a low molecular weight protein which in the reconstituted system increases the Vmax of cleavage for both substrates but does not affect the Km of either. Side-chain cleavage activity was also found in bovine adrenocortical cytosol and this appears to differ from that present in mitochondria.

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References

    1. J Biol Chem. 1979 Jan 25;254(2):547-52 - PubMed
    1. J Biol Chem. 1979 Apr 25;254(8):2766-74 - PubMed
    1. J Biol Chem. 1968 Oct 10;243(19):5153-7 - PubMed
    1. J Biol Chem. 1970 Jan 10;245(1):152-9 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed

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