doi: 10.1073/pnas.80.7.1955.
Novel B family sequence from an early chorion cDNA library of Bombyx mori
- PMID: 6572954
- PMCID: PMC393730
- DOI: 10.1073/pnas.80.7.1955
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Novel B family sequence from an early chorion cDNA library of Bombyx mori
Proc Natl Acad Sci U S A.
1983 Apr.
Abstract
A cDNA library has been constructed from transcripts present during the early stages of choriogenesis in the silk-moth, Bombyx mori. Clones representing novel sequences were selected by screening the library with (i) homotypic cDNA, (ii) rDNA and typical chorion probes of the A and B multigene families and (iii) selected clones from the library, which were positive in step (i) but negative in step (ii); this third step identified clones that hybridized to distinct subsets of the library. The sequence of one such clone was determined and shown to represent a novel subfamily of the B chorion multigene family, exclusively expressed during the early choriogenic period.
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