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. 1983 Jun;80(12):3661-5.
doi: 10.1073/pnas.80.12.3661.

Primary structure of chicken muscle pyruvate kinase mRNA

N Lonberg, W Gilbert

Primary structure of chicken muscle pyruvate kinase mRNA

N Lonberg et al. Proc Natl Acad Sci U S A. 1983 Jun.

Abstract

We have determined the cDNA sequence corresponding to chicken muscle pyruvate kinase mRNA; the predicted coding region spans 529 amino acids and establishes the complete amino acid sequence for the vertebrate enzyme. We demonstrate that the level of mRNA for this enzyme is under developmental control and suggest a structural model for the protein kinase-mediated regulation of the mammalian liver isozyme. We report a method for the direct analysis of, and the preparation of cDNA probes from, mRNA which has been fractionated on methylmercury/agarose gels.

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