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. 1983 Jul;80(13):3963-5.
doi: 10.1073/pnas.80.13.3963.

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination

M D Biggin, T J Gibson, G F Hong

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination

M D Biggin et al. Proc Natl Acad Sci U S A. 1983 Jul.

Abstract

Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation on an autoradiograph, allows more sequence to be obtained from a gel. We also show that the use of deoxyadenosine 5'-(alpha-[35S]thio)triphosphate as the label incorporated in dideoxynucleotide sequence reactions increases the sharpness of the bands on an autoradiograph and so increases the resolution achieved.

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