doi: 10.1073/pnas.81.2.304.
Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences
- PMID: 6582488
- PMCID: PMC344664
- DOI: 10.1073/pnas.81.2.304
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Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences
Proc Natl Acad Sci U S A.
1984 Jan.
Abstract
Nuclear matrices containing residual DNA were isolated from chicken erythrocytes after extraction of purified nuclei with buffered 2 M NaCl. After further purification of this residual DNA, it was found to contain high concentrations of beta-globin gene sequences as assayed by dot hybridization with 32P-labeled nick-translated pHB1001. Electron microscopy of a random sample of this residual DNA fraction shows the DNA to be intimately associated with protein at various intervals. A hypothesis for enrichment of active genes in residual DNA from purified chromatin or in nuclear matrix DNA is also discussed.
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