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. 1984 Jan;81(2):357-61.
doi: 10.1073/pnas.81.2.357.

Purification and characterization of acidic fibroblast growth factor from bovine brain

K A Thomas, M Rios-Candelore, S Fitzpatrick

Purification and characterization of acidic fibroblast growth factor from bovine brain

K A Thomas et al. Proc Natl Acad Sci U S A. 1984 Jan.

Abstract

Acidic brain fibroblast growth factor has been purified a minimum of 35,000-fold to apparent homogeneity by a combination of differential salt precipitation, ion exchange chromatography, gel filtration, isoelectric focusing, and hydrophobic chromatography on a C4 reversed-phase HPLC column. Two microheterogeneous forms of the molecule are obtained with apparent molecular masses of 16,600 and 16,800 daltons. The mitogen is highly active with half-maximal stimulation of BALB/c 3T3 fibroblasts at about 40 pg/ml in an assay using incorporation of [methyl-3H]thymidine into DNA.

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