A longitudinal study of the prostaglandin content of central nervous system tissues from guinea pigs with acute experimental allergic encephalomyelitis (EAE)

Abstract

The levels of prostaglandins (PGs) E, F2 alpha and 6-oxo-PGF1 alpha, in spinal cords and cerebellums of guinea pigs were measured during the development of experimental allergic encephalomyelitis (EAE). The earliest change observed was an elevation of PGE in spinal cords, but not cerebellums , 5-7 days post-inoculation (PI) and prior to the appearance of clinical symptoms. PGE content of spinal cords continued to rise until days 12-14 PI when the animals displayed paralytic EAE. In contrast, PGF2 alpha and 6-oxo-PGF1 alpha levels in spinal cords peaked on days 9-11, when the animals exhibited initial clinical signs, but fell to lower values by days 12-14 PI. In cerebellums , the PGE content increased more slowly than in spinal cords, consistent with the lower numbers of mononuclear cell infiltrates, whereas PGF2 alpha and 6-oxo-PGF1 alpha levels remained unaltered. The relationships between the observed changes in prostanoid levels, lesion development and the appearance of clinical symptoms are discussed.