Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Jan-Feb;86(1 Pt 2 Suppl 35):1-8.
doi: 10.1177/00034894770860s101.

An improved method for processing early rat otocysts for the electron microscope

T Schulte, K M Khan, W F Marovitz

An improved method for processing early rat otocysts for the electron microscope

T Schulte et al. Ann Otol Rhinol Laryngol Suppl. 1977 Jan-Feb.

Abstract

Various methods of dissection, fixation, osmication, sectioning and staining were tested in order to develop an acceptable technique for preparing 9, 10, 11, and 12-day-old rat otocysts for electron microscopic study. The general problems associated with embryonic tissue-difficult handling, high water content and poor stainability are discussed, and concrete methods of preparation which significantly decrease these difficulties are proposed. The specific fixation and sectioning requirements of rat otocyst are also described in the elaboration of a method which will be used in subsequent studies of the organogenesis of rodent ear.

PubMed Disclaimer