Differential protein insertion into developing photosynthetic membrane regions of Rhodopseudomonas sphaeroides

Abstract

Previous studies have suggested that much of the B800-850 light-harvesting bacteriochlorophyll a-protein complex is inserted directly into the intracytoplasmic photosynthetic membrane of Rhodopseudomonas sphaeroides. In contrast, the B875 light-harvesting and reaction center complexes are assembled preferentially at peripheral sites of photosynthetic membrane growth initiation. The basis for this apparent site-specific polypeptide insertion was examined during the inhibition of RNA and protein syntheses. The pulse labeling of polypeptides at the membrane growth initiation sites was significantly less sensitive to inhibition by rifampicin, chloramphenicol, or kasugamycin than in the intracytoplasmic or outer membranes. This suggests increased stability for the translation machinery at these membrane invagination sites. Similar differential effects in polypeptide insertion were observed during inhibition of bacteriochlorophyll synthesis through deprival of delta-aminolevulinate to R sphaeroides mutant H-5, which requires this porphyrin precursor. The pulse-labeling patterns observed during the inhibition of both RNA and pigment syntheses were consistent with the uncoupling of polypeptide insertion into the membrane invagination sites from their growth and maturation into intracytoplasmic membranes.