Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1983 Jun;131(2):341-4.
doi: 10.1016/0003-2697(83)90180-x.

A spectrophotometric assay for dehydroascorbate reductase

R L Stahl, L F Liebes, C M Farber, R Silber
Comparative Study

A spectrophotometric assay for dehydroascorbate reductase

R L Stahl et al. Anal Biochem. 1983 Jun.

Abstract

A simple spectrophotometric assay for dehydroascorbate reductase based on the change in absorbance associated with the formation of ascorbic acid is described. Using a partially purified preparation from spinach leaves, the reaction was found to be linear with time and enzyme concentration. The reaction rate determined by this assay correlated well with that obtained by a high-performance liquid chromatography method. Possible advantages over currently available assays as well as potential applications are discussed.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources