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. 1983 Jun;131(2):373-8.
doi: 10.1016/0003-2697(83)90186-0.

Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins

H Beinert

Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins

H Beinert. Anal Biochem. 1983 Jun.

Abstract

Details are provided for a reproducible procedure for determination of labile sulfide in iron-sulfur (Fe-S) proteins in the range of 1 to 3 nmol. Analyses are also presented on the most stable Fe-S protein so far reported. In this case denaturation with guanidine.HCl was used in the presence of dithiothreitol. The values obtained then also include any sulfane sulfur (S0) present.

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