doi: 10.1093/oxfordjournals.jbchem.a134350.
A new method for purification of anti-glycosphingolipid antibody. Avian anti-hematoside (NeuGc) antibody
- PMID: 6619119
- DOI: 10.1093/oxfordjournals.jbchem.a134350
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A new method for purification of anti-glycosphingolipid antibody. Avian anti-hematoside (NeuGc) antibody
J Biochem.
1983 Jul.
Free article
Abstract
A new method for purification of anti-glycosphingolipid antibodies had been developed. N-Glycolylneuraminyl(alpha 2-3)lactosylceramide [hematoside (NeuGc)] could be hydrophobically bound on octyl-Sepharose 4B in the presence of 0.1 M KCl. The Sepharose gel coated with hematoside (NeuGc) was used as immunoadsorbent for affinity column chromatography to purify avian anti-hematoside (NeuGc) antibody. The procedure is very simple, reproducible and applicable to purification of almost all anti-glycosphingolipid antibodies. The glycosphingolipid used for the affinity chromatography could be recovered without any destruction by successive extraction of the gel with methanol and methanol/chloroform (1:2, v/v).
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