Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Sep 15;214(3):959-65.
doi: 10.1042/bj2140959.

A critical appraisal of the effect of oxidized glutathione on hepatic glucose 6-phosphate dehydrogenase activity

H R Levy, M Christoff

A critical appraisal of the effect of oxidized glutathione on hepatic glucose 6-phosphate dehydrogenase activity

H R Levy et al. Biochem J. .

Abstract

Experiments were undertaken to elucidate the mechanism of the reversal of NADPH inhibition of rat liver glucose 6-phosphate dehydrogenase by oxidized gluthathione alone and in combination with a putative cofactor described by Eggleston & Krebs [(1974) Biochem. J. 138, 425-435]. Evidence is presented that this reversal is largely an artifact, caused by the incorrect application of a control assay procedure and a spurious effect of Zn2+ (added in order to inhibit glutathione reductase) in crude enzyme solutions. When the proper assay procedure is used and glutathione reductase is inhibited with low concentrations of Hg2+, glutathione addition has no effect on NADPH inhibition of glucose 6-phosphate dehydrogenase. No evidence was found for the existence of a cofactor that mediates an effect of glutathione on glucose 6-phosphate dehydrogenase.

PubMed Disclaimer

References

    1. J Biol Chem. 1980 Jun 25;255(12):5616-26 - PubMed
    1. Biochemistry. 1982 Dec 21;21(26):6628-33 - PubMed
    1. Arch Biochem Biophys. 1974 Nov;165(1):398-406 - PubMed
    1. Biochem J. 1974 Mar;138(3):425-35 - PubMed
    1. Biochem J. 1978 Mar 15;170(3):577-82 - PubMed

Publication types