Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Sep;18(3):469-71.
doi: 10.1128/jcm.18.3.469-471.1983.

Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood

J Bille, L Stockman, G D Roberts, C D Horstmeier, D M Ilstrup

Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood

J Bille et al. J Clin Microbiol. 1983 Sep.

Abstract

A lysis-centrifugation system (Isolator; E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with a biphasic brain heart infusion (BHI) medium in a prospective study of 5,125 fungal blood cultures. The Isolator recovered 90.3% of the positive cultures, compared with 63.4% recovered by the biphasic BHI medium. Overall, the detection of fungemia was increased 36.6% by the Isolator. Mean recovery times for yeasts were 2.12 and 4.90 days for the Isolator and BHI bottles, respectively. Cultures of Histoplasma capsulatum required 8.0 and 24.14 days for recovery by the Isolator and BHI bottles, respectively. The Isolator provided a more rapid and sensitive means of detecting organisms associated with fungemia.

PubMed Disclaimer

References

    1. Lancet. 1974 Nov 16;2(7890):1174-6 - PubMed
    1. J Clin Microbiol. 1975 Feb;1(2):154-6 - PubMed
    1. J Clin Microbiol. 1975 Jul;2(1):18-20 - PubMed
    1. J Clin Microbiol. 1979 Mar;9(3):391-6 - PubMed
    1. J Clin Microbiol. 1981 Dec;14(6):681-3 - PubMed