Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing
- PMID: 6630448
- PMCID: PMC270883
- DOI: 10.1128/jcm.18.3.722-724.1983
Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing
Abstract
The detection of thermonuclease activity in 86 blood culture samples containing gram-positive cocci showed 100% correlation with the subsequent identification of the isolate as Staphylococcus aureus by the coagulase test. No positive thermonuclease results were found with 66 samples containing coagulase-negative staphylococci and 56 samples containing other gram-positive organisms. The thermonuclease test provides a rapid, reliable method to identify S. aureus in blood cultures.
Similar articles
-
Thermonuclease test for same-day identification of Staphylococcus aureus in blood cultures.J Clin Microbiol. 1985 Jun;21(6):995-6. doi: 10.1128/jcm.21.6.995-996.1985. J Clin Microbiol. 1985. PMID: 4008628 Free PMC article.
-
A comparison of the rapid thermonuclease test and the lysostaphin susceptibility test in the presumptive identification of Staphylococcus aureus from positive Bactec blood cultures.Diagn Microbiol Infect Dis. 1989 May-Jun;12(3):205-9. doi: 10.1016/0732-8893(89)90016-3. Diagn Microbiol Infect Dis. 1989. PMID: 2676326
-
Use of thermonuclease testing to identify Staphylococcus aureus by direct examination of blood cultures.East Mediterr Health J. 2003 Jan-Mar;9(1-2):185-90. East Mediterr Health J. 2003. PMID: 15562750
-
Clinical laboratory evaluation of the thermonuclease test.Am J Clin Pathol. 1982 May;77(5):587-91. doi: 10.1093/ajcp/77.5.587. Am J Clin Pathol. 1982. PMID: 6805307
-
Ultrasensitive detection of micrococcal nuclease activity and Staphylococcus aureus contamination using optical biosensor technology-A review.Talanta. 2021 May 1;226:122168. doi: 10.1016/j.talanta.2021.122168. Epub 2021 Jan 30. Talanta. 2021. PMID: 33676710 Review.
Cited by
-
Evaluation of LAMP Assay Using Phenotypic Tests and Conventional PCR for Detection of nuc and mecA genes Among Clinical Isolates of Staphylococcus spp.J Clin Diagn Res. 2015 Aug;9(8):DC06-9. doi: 10.7860/JCDR/2015/13962.6315. Epub 2015 Aug 1. J Clin Diagn Res. 2015. PMID: 26435941 Free PMC article.
-
Accuracy of PCR targeting different markers for Staphylococcus aureus identification: a comparative study using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as the gold standard.J Vet Diagn Invest. 2018 Mar;30(2):252-255. doi: 10.1177/1040638717732370. Epub 2017 Nov 6. J Vet Diagn Invest. 2018. PMID: 29105571 Free PMC article.
-
Thermonuclease test for same-day identification of Staphylococcus aureus in blood cultures.J Clin Microbiol. 1985 Jun;21(6):995-6. doi: 10.1128/jcm.21.6.995-996.1985. J Clin Microbiol. 1985. PMID: 4008628 Free PMC article.
-
Thermonuclease test accuracy is preserved in methicillin-resistant Staphylococcus aureus isolates.J Med Microbiol. 2020 Apr;69(4):548-551. doi: 10.1099/jmm.0.001166. J Med Microbiol. 2020. PMID: 32101159 Free PMC article.
-
Molecular docking and proteomics reveals the synergistic antibacterial mechanism of theaflavin with β-lactam antibiotics against MRSA.Front Microbiol. 2022 Nov 14;13:993430. doi: 10.3389/fmicb.2022.993430. eCollection 2022. Front Microbiol. 2022. PMID: 36452924 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources