doi: 10.1016/0090-6980(78)90122-3.
A radioimmunoassay for 6-keto-prostaglandin F1alpha
- PMID: 663275
- DOI: 10.1016/0090-6980(78)90122-3
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A radioimmunoassay for 6-keto-prostaglandin F1alpha
Prostaglandins.
1978 Mar.
Abstract
A radioimmunoassay for 6-keto-prostaglandin F1alpha has been developed. The assay is accurate and sensitive but since the antiserum cross-reacts 5-10% with prostaglandins (PGs) of the E and F series, solvent extraction and thin layer chromatography are required for absolute specificity. The assay has been validated by comparison with a radiochemical assay and by the use of an inhibitor of 6-keto PGF1alpha formation, 15-hydroperoxy arachidonic acid. 6-Keto PGF1alpha was found to have a low cross reaction with antisera directed against PGE2, PGF2alpha and thromboxane B2.
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