Methods for the determination of lorazepam and chlordiazepoxide and metabolites in brain tissue. A comparison with plasma concentrations in the rat
- PMID: 6643606
- DOI: 10.1016/s0378-4347(00)84837-9
Methods for the determination of lorazepam and chlordiazepoxide and metabolites in brain tissue. A comparison with plasma concentrations in the rat
Abstract
Rapid and sensitive methods are described for determining lorazepam and for determining chlordiazepoxide and its metabolites in brain tissue of the rat. Lorazepam was determined by means of solvent extraction and electron-capture gas-liquid chromatography and concentrations as low as 5 ng/g tissue could be measured. High-performance liquid chromatography with UV detection was used to determine chlordiazepoxide and its metabolites and was sensitive to 0.1 micrograms/g tissue. The methods were used to investigate the brain and plasma pharmacokinetics of these compounds in animals that had been chronically treated with lorazepam or chlordiazepoxide. In both experiments brain and plasma levels of all compounds assayed were found to correlate highly.
Similar articles
-
Pharmacokinetic studies on Ro 15-1788, a benzodiazepine receptor ligand, in the brain of the rat.Brain Res. 1984 Jan 2;290(1):183-6. doi: 10.1016/0006-8993(84)90752-2. Brain Res. 1984. PMID: 6419985
-
Micro-extraction of chlordiazepoxide and its primary metabolites, desmethylchlordiazepoxide and demoxepam, from plasma and their measurement by liquid chromatography.Ann Clin Biochem. 1998 Jul;35 ( Pt 4):528-33. doi: 10.1177/000456329803500407. Ann Clin Biochem. 1998. PMID: 9681055
-
Analysis of lorazepam in rat brain using liquid/liquid and solid-phase extraction in combination with high performance liquid chromatography.Biomed Chromatogr. 1990 Jul;4(4):168-70. doi: 10.1002/bmc.1130040412. Biomed Chromatogr. 1990. PMID: 2207380
-
Is chlordiazepoxide the rational choice among benzodiazepines?Pharmacotherapy. 1982 Mar-Apr;2(2):110-9. doi: 10.1002/j.1875-9114.1982.tb03181.x. Pharmacotherapy. 1982. PMID: 6152590 Review.
-
Chromatography of the 1,4-benzodiazepines.J Chromatogr. 1974 Oct 30;98(3):527-68. doi: 10.1016/s0021-9673(00)84071-6. J Chromatogr. 1974. PMID: 4153870 Review. No abstract available.
Cited by
-
The role of the benzodiazepine receptor in mediating long-lasting anticonvulsant effects and the late-appearing reductions in motor activity and exploration.Psychopharmacology (Berl). 1989;97(3):349-54. doi: 10.1007/BF00439449. Psychopharmacology (Berl). 1989. PMID: 2541459
-
Long-lasting anticonvulsant effects of diazepam in different mouse strains: correlations with brain concentrations and receptor occupancy.Psychopharmacology (Berl). 1985;86(1-2):137-41. doi: 10.1007/BF00431698. Psychopharmacology (Berl). 1985. PMID: 3927350
-
A pharmacokinetic study of CGS-8216, a benzodiazepine receptor ligand, in the rat.Psychopharmacology (Berl). 1984;84(3):420-2. doi: 10.1007/BF00555224. Psychopharmacology (Berl). 1984. PMID: 6096912
-
Changes in seizure threshold and aggression during chronic treatment with three anticonvulsants and on drug withdrawal.Psychopharmacology (Berl). 1990;100(2):237-42. doi: 10.1007/BF02244413. Psychopharmacology (Berl). 1990. PMID: 2305012
-
Evidence of strain differences in GABA-benzodiazepine coupling.Psychopharmacology (Berl). 1987;93(1):127-32. doi: 10.1007/BF02439599. Psychopharmacology (Berl). 1987. PMID: 2819923