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. 1983 Nov 11;64(1-2):39-43.
doi: 10.1016/0022-1759(83)90382-4.

Dot-blotting--a novel screening assay for antibodies in hybridoma cultures

J Sternberg, P Jeppesen

Dot-blotting--a novel screening assay for antibodies in hybridoma cultures

J Sternberg et al. J Immunol Methods. .

Abstract

A novel solid phase radioimmunoassay using nitrocellulose filters, is described. The method is of particular value in screening hybridoma cultures for monoclonal antibodies against chromosomal protein. Also it permits detection of antibodies directed against epitopes stable to SDS denaturation.

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