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Comparative Study
. 1983 Nov;141(3):263-8.
doi: 10.1620/tjem.141.263.

A new mass screening method for determining UDP-galactose in blood

Y Fujimura, M Kawamura, H Naruse
Free article
Comparative Study

A new mass screening method for determining UDP-galactose in blood

Y Fujimura et al. Tohoku J Exp Med. 1983 Nov.
Free article

Abstract

We devised a new microfluorometric mass screening method for determining UDP-galactose by using only one blood disc of 3 mm in diameter. This method contains two coupling enzymes of UDP-galactose-4-epimerase and UDP-glucose dehydrogenase, and conversion of NAD to NADH. The assay ranges are 0 to 1 mM or 0 to 0.2 mM UDP-galactose in blood. UDP-galactose contents were determined in galactosemia of epimerase-, transferase- and kinase-deficiencies, and several cases with high or low Paigen's values.

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