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. 1983 Nov 15;116(3):1000-6.
doi: 10.1016/s0006-291x(83)80241-1.

Preparation of a covalently linked adduct between plastocyanin and cytochrome f

D J Davis, K Hough

Preparation of a covalently linked adduct between plastocyanin and cytochrome f

D J Davis et al. Biochem Biophys Res Commun. .

Abstract

Plastocyanin can be covalently cross-linked to the monomeric cytochrome f from turnip by incubation in the presence of a water-soluble carbodiimide. The adduct between the two proteins has a molecular weight of approximately 43,000 suggesting a 1:1 stoichiometry between the two proteins of the adduct. This stoichiometry has been verified by spectral characterization of the adduct. The efficiency of the cross-linking reaction is pH dependent with a higher degree of cross-linking being observed at pH 6.5 than at pH 7.0.

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