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Comparative Study
. 1983 Nov 30;135(1):73-82.
doi: 10.1016/0009-8981(83)90390-x.

A novel combination of techniques for the assay of lactate dehydrogenase isoenzymes in plasma and red blood cell lysates

I Hunter, B Attock, T Palmer
Comparative Study

A novel combination of techniques for the assay of lactate dehydrogenase isoenzymes in plasma and red blood cell lysates

I Hunter et al. Clin Chim Acta. .

Abstract

A method is presented for the preparation and assay of lactate dehydrogenase isoenzymes 1 and 2 as pure fractions. The total method involves the use of heat treatment to destroy the heat-labile fractions (isoenzymes 3, 4 and 5); fractionation using an ion-exchange resin; and immunochemical blocking of the muscle subunit, to remove any contamination of the fraction containing isoenzyme 1 with isoenzyme 2 and vice versa. Finally, the enzyme activities in the various fractions are measured using a standard kinetic assay procedure. Estimates can be obtained of all five isoenzymes if the heat treatment step is omitted.

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