Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Dec;44(3):315-24.
doi: 10.1016/S0006-3495(83)84305-7.

A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution

E Gratton, M Limkeman

A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution

E Gratton et al. Biophys J. 1983 Dec.

Abstract

A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported. The phase fluorometer operates over the frequency range 1-160 MHz with a maximum resolution of a few picoseconds. The effects of distortions introduced by the light modulator and the nonlinear dynode characteristic are discussed in terms of the harmonic content of the detected signal. A source of systematic errors due to nonhomogeneous modulation is also discussed with particular attention to the color effect of the photomultipliers. The application of the phase fluorometer to the measurement of very long and very short lifetimes is reported. Some application to the measurement of multiexponential decays is also illustrated.

PubMed Disclaimer

References

    1. J Biochem Biophys Methods. 1981 Sep;5(3):131-46 - PubMed

Publication types