Identification of simian virus 40 tumor and U antigens

Abstract

The synthesis and identity of the tumor and U antigens of simian virus 40 (SV 40) have been examined during productive infection in monkey cells, abortive infection in mouse cells, and in SV40-transformed mouse cells by using sodium dodecyl sulfate/polyacrylamide slab gel electrophoresis to analyze [35S]methionine-labeled radioimmune precipitates. The following observations were made: (i) the tumor and U antigenic sites are on the same 94,000, 89,000, and 84,000 molecular weight species detected during productive infection; a 94,000 species made during abortive infection; and a 94,000 species found in transformed cells. (ii) The 94,000 species is relatively unstable compared to the relatively stable 89,000 and 84,000 species produced during productive infection. (iii) The stable 89,000 and 84,000 molecular weight species are differentially extracted from productively infected cells, which suggests an intracellular compartmentation and/or different affinities of these species for cellular substrates. (iv) The 94,000 species synthesized during abortive infection is more stable than the comparable 94,000 species synthesized in transformed cells. (v) Three tsA group mutants overproduce several unstable species of tumor antigen at restrictive temperature.