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. 1978 Jun 9;524(2):254-61.
doi: 10.1016/0005-2744(78)90162-6.

Rapid affinity purification and properties of rat liver sorbitol dehydrogenase

N Leissing, E T McGuinness

Rapid affinity purification and properties of rat liver sorbitol dehydrogenase

N Leissing et al. Biochim Biophys Acta. .

Abstract

A 23-h affinity chromatography purification procedure for sorbitol dehydrogenase (L-iditol:NADl-oxidoreductase, EC 1.1.1.14) prepared from freshly excised rat liver has been developed that resulted in an 18% yield of an apparently homogeneous preparation (purification = 439-fold). The molecular weight of the enzyme was approx. 96 000. The enzyme was specific for NAD+ (NADH), but had no requirement for NADP+ (NADPH). The purified preparation shows significant activity with structurally related polyols and ketoses. Km values for sorbitol and fructose are 0.35 and 110 mM (at pH 7.1), respectively.

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