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. 1983 Mar;1(1):59-63.
doi: 10.1016/0732-8893(83)90033-0.

Improved immunologic detection of Clostridium difficile antigen by counterimmunoelectrophoresis

R W Ryan, I Kwasnik, R C Tilton

Improved immunologic detection of Clostridium difficile antigen by counterimmunoelectrophoresis

R W Ryan et al. Diagn Microbiol Infect Dis. 1983 Mar.

Abstract

An improved counterimmunoelectrophoresis (CIE) procedure for detection of Clostridium difficile antigen is described. Commercially available antiserum to C. difficile toxin was absorbed with whole cells of C. difficile. CIE (absorbed) was 100% sensitive and 77.5% specific when compared to the tissue culture toxin assay. Instances are noted in which the CIE (absorbed) and/or bacterial culture was positive and the tissue culture assay was negative.

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