Post-transcriptional processing of simian virus 40 late transcripts in injected frog oocytes

Abstract

The capacity of fully grown Xenopus oocytes to process messenger RNA precursors has been assessed using transcripts synthesized from simian virus 40 (SV40) DNA microinjected into the oocyte nucleus. In oocytes, stable transcripts of the SV40 virion protein genes have undergone at least four post-transcriptional maturation steps: cleavage at 3' splice sites, formation of a mature 3' terminus, addition of poly(A), and selective intracellular partitioning, such that only those RNAs with a mature 3' terminus and poly(A) are located in the cytoplasm. Apparently unspliced transcripts with mature 3' termini are transported into the oocyte cytoplasm. A prominent transcript of roughly the full length of SV40 DNA, bearing a 5' terminus in the same region as late mRNA and confined to the nucleus, is found in oocytes injected with SV40 DNA. The possibility that this transcript may serve as a precursor to late mRNA is discussed.