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Comparative Study
. 1983 Mar:20 Pt 2:112-5.
doi: 10.1177/000456328302000210.

Comparison of Bolton-Hunter and chloramine-T techniques for the radioiodination of prostatic acid phosphatase

C M Sturgeon, L Beynon, J Seth, G D Chisholm
Comparative Study

Comparison of Bolton-Hunter and chloramine-T techniques for the radioiodination of prostatic acid phosphatase

C M Sturgeon et al. Ann Clin Biochem. 1983 Mar.

Abstract

A double antibody, semi-automated radioimmunoassay for serum prostatic acid phosphatase (PAP) is described, which uses the 125I-labelled N-succinimidyl-3-(4-hydroxyphenyl) propionate ester of PAP. This type of label has substantially higher immunoreactivity than that prepared using the chloramine-T method of radioiodination. Chromatographic purification of either label on Ultrogel ACA44 further improved immunoreactivity. The lowest detection limit (0 . 35 micrograms/l) was achieved with the chromatographically purified ester label.

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