Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Apr 30;126(2):669-77.
doi: 10.1016/s0042-6822(83)80022-1.

Coronaviruses SD and SK share extensive nucleotide homology with murine coronavirus MHV-A59, more than that shared between human and murine coronaviruses

S R Weiss

Coronaviruses SD and SK share extensive nucleotide homology with murine coronavirus MHV-A59, more than that shared between human and murine coronaviruses

S R Weiss. Virology. .

Abstract

A cDNA probe representing the genome of mouse hepatitis virus (MHV) strain A59 (MHV-A59) was used to measure nucleotide sequence homologies among murine and human coronaviruses and the SD and SK coronaviruses isolated by Burks et al. Since SD and SK were isolated by inoculation of multiple sclerosis (MS) central nervous system (CNS) tissue into mice or cultured mouse cells, it is important to determine their relationships to other murine and human coronavirus isolates. Our results indicate that SD and SK share almost complete nucleotide homology (approximately 90%) with MHV-A59 and generate subgenomic RNAs of the same sizes as MHV-A59. The human coronavirus (HCV) strains tested show less homology with MHV-A59. The immunologically unrelated HCV-229E shows no nucleotide homology with MHV-A59. The immunologically cross-reactive HCV-OC43 shows nucleotide homology with MHV-A59 by blot hybridization but not when hybridized in solution and assayed by S1 nuclease digestion.

PubMed Disclaimer

References

    1. Alwine J.C., Kemp D.J., Stark G.R. Vol. 14. 1977. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl paper and hybridization with DNA probes; pp. 5350–5354. (Proc. Nat. Acad. Sci. USA). - PMC - PubMed
    1. Bailey J., Davidson N. Methylmercury as a reversible denaturing agent for agarose-gel electrophoresis. Anal. Biochem. 1976;70:75–85. - PubMed
    1. Burks J.S., DeVald B.L., Jankovsky L.D., Gerdes J.C. Two coronaviruses isolated from central nervous system tissue of two multiple sclerosis patients. Science. 1980;209:933–934. - PubMed
    1. Chamberlain J.P. Fluorographic detection of radioactivity in polyacrylamide gels with the water soluble fluor, sodium salicylate. Anal. Biochem. 1979;48:132–135. - PubMed
    1. Cheley S., Anderson R., Cupples M.J., Lee Chan E.C.M., Morris V.L. Intracellular murine hepatitis virus-specific RNAs contain common sequences. Virology. 1981;112:596–604. - PMC - PubMed

Publication types

LinkOut - more resources