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. 1983 Nov 11;11(21):7427-34.
doi: 10.1093/nar/11.21.7427.

Isolation and characterisation of cDNA clones for the A alpha- and gamma-chains of human fibrinogen

A M Imam, M A Eaton, R Williamson, S Humphries
Free PMC article

Isolation and characterisation of cDNA clones for the A alpha- and gamma-chains of human fibrinogen

A M Imam et al. Nucleic Acids Res. .
Free PMC article

Abstract

cDNA clones coding for the A alpha- and gamma-chains of human fibrinogen have been isolated from an adult liver cDNA library. Clones were identified by hybridisation with mixtures of synthetic oligonucleotides 17 bases long, predicted using amino acid sequence data for each chain. The cDNA insert sizes are 1,950bp for A alpha-fibrinogen and 950bp for gamma-fibrinogen. The clones do not show any cross-hybridisation. Each cDNA hybridises to a unique sequence in the human genome. In adult human liver, Northern blots give an estimated messenger RNA size of 2.6kb for A alpha-fibrinogen and 1.8kb for gamma-fibrinogen.

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References

    1. Thromb Res. 1976 May;8(5):639-58 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Hoppe Seylers Z Physiol Chem. 1977 Jul;358(7):935-8 - PubMed
    1. Br Med Bull. 1977 Sep;33(3):283-8 - PubMed
    1. Cell. 1977 Oct;12(2):429-39 - PubMed

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