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. 1984 Feb;150(2):477-82.
doi: 10.1016/0014-4827(84)90592-5.

Electrofusion. A new, highly efficient technique for generating somatic cell hybrids

C Finaz, A Lefevre, J Teissié

Electrofusion. A new, highly efficient technique for generating somatic cell hybrids

C Finaz et al. Exp Cell Res. 1984 Feb.

Abstract

A rapid and highly efficient method for generating viable somatic cell hybrids is described. A co-culture of clone ID and CH rodent cell lines was exposed to five successive electric pulses of about 1.5 kV/cm with a duration of 50 musec. This treatment induced extensive cell fusion, and independent hybrid clones were generated with a frequency of 1 X 10(-3), which represents a 100-fold increase over the polyethylene glycol induced fusion. Seventeen of them were propagated in selective medium, karyotyped and analysed for their enzyme markers, in order to establish their hybrid nature.

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