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. 1984;65(4):391-5.
doi: 10.1007/BF00291565.

Detailed cell cycle analysis in human lymphocytes; application to gamma-irradiated cells

J Couturier, J L Antoine

Detailed cell cycle analysis in human lymphocytes; application to gamma-irradiated cells

J Couturier et al. Hum Genet. 1984.

Abstract

A method based on BrdU incorporation for analyzing in detail the kinetics of the cell cycle is described. The S phase has been subdivided into five subphases, each recognizable by their BrdU incorporation pattern at metaphase. The method can be useful for the study of abnormal cell cycles, and may have particular application in mutagenesis studies concerning the various subphases of the S phase, without using synchronization techniques. An application of the method is described, showing that gamma-irradiation, during the course of the S phase, leads to a lack of cells which were in early S phase at the time of irradiation. This finding can be related either to a higher lethality at this stage of the cell cycle or to a delay in completion of DNA replication after irradiation.

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References

    1. Chromosoma. 1975 Dec 10;53(3):209-21 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3395-9 - PubMed
    1. Radiat Environ Biophys. 1977 Dec 12;14(4):285-94 - PubMed
    1. Hum Genet. 1978 Aug 31;43(2):139-49 - PubMed
    1. Ann Genet. 1976 Jun;19(2):95 - PubMed

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