Suramin effects on macrophage phagolysosome formation and antimicrobial activity

Abstract

The effects of suramin on phagolysosome formation and antimicrobial activity of mouse peritoneal macrophages cultivated in vitro have been studied. Prolonged in vitro pretreatment of macrophages with high concentrations of suramin caused macrophages to form large fragile phagolysosomes in which the concentrations of the various lysosomal enzymes were inferred to be diminished. In addition, suramin-treated macrophages demonstrated enhanced exocytosis of acid phosphatase during phagocytosis of polyvinyl toluene spherules. However, suramin was found not to inhibit formation of phagolysosomes in macrophages that had ingested Listeria monocytogenes when those cells were examined by the electron microscope. Suramin pretreatment did not alter the ingestion or intracellular killing of Staphylococcus aureus or of a strain of L. monocytogenes that was essentially avirulent for mice, but did protect macrophages from destruction by virulent L. monocytogenes ingested in vitro, an effect that appeared to have been mediated through enhancement of the bacteriostatic potential of the macrophages. However, at a single dosage level, the drug did not alter the mortality of mice challenged with virulent L. monocytogenes.