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. 1984 Jan-Feb;67(1):32-4.

Preparation of deoxynivalenol (vomitoxin) from field-inoculated corn

P M Scott, G A Lawrence, A Telli, J R Iyengar
  • PMID: 6698925

Preparation of deoxynivalenol (vomitoxin) from field-inoculated corn

P M Scott et al. J Assoc Off Anal Chem. 1984 Jan-Feb.

Abstract

A process was developed for production of gram quantities of deoxynivalenol (DON) from corn that had been inoculated in the field with Fusarium graminearum and was estimated to contain 400-500 mg DON/kg. Steps in the purification procedure included extraction with methanol-water (1 + 1), partition from an aqueous solution into ethyl acetate by using a hydrophilic matrix, defatting, Florisil column chromatography, methylene chloride-water partition, semipreparative liquid chromatography (LC), and crystallization from ethyl acetate. The average yield of crystalline DON produced by this method (or with minor variations) was 281 mg/kg.

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