Possible projections from regions of paraventricular and supraoptic nuclei to the spinal cord: electrophysiological studies

Abstract

The existence of monosynaptic connections between neurons in the paraventricular nucleus (PVN) of the hypothalamus and the intermediolateral cell column (ILC) of the spinal cord was studied by electrophysiological techniques in chloralose-anesthetized cats. Sympathetic preganglionic discharges (recorded from the 2nd or 3rd thoracic white ramus) were evoked by microstimulation of certain regions in or near the PVN with short train of pulses and below 50 microA current. By recording responses of 'identified' and 'non-identified' neurosecretory cells in the PVN and supraoptic nucleus (SON) to stimulation of the ILC of the thoracic cord, it was possible to identify antidromically evoked action potentials in 9 out of 297 neurons tested. Among them, 2 neurons were also antidromically excited by the pituitary stalk stimulation, 5 were orthodromically excited by the same stimulus and the remaining 2 were not excited by the stalk stimulation. Our results indicate that some PVN neurons, though small in number, send axons directly to the ILC of the cord, and that a very few neurons among these also send their axons to the pituitary gland.