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. 1984 Feb 15;218(1):269-72.
doi: 10.1042/bj2180269.

Cleavage of formate from omega,4-dihydroxyacetophenone. An unusual oxygen-requiring reaction in the bacterial catabolism of 4-hydroxyacetophenone

D J Hopper, E A Elmorsi

Cleavage of formate from omega,4-dihydroxyacetophenone. An unusual oxygen-requiring reaction in the bacterial catabolism of 4-hydroxyacetophenone

D J Hopper et al. Biochem J. .

Abstract

An enzyme, from a soil bacterium grown on 4-hydroxyacetophenone, cleaved the side chain of omega,4-dihydroxyacetophenone between the keto group and the carbon atom bearing a hydroxy group to give 4-hydroxybenzoate and formate. The reaction was O2-dependent. Partially purified enzyme required no added cofactors for activity.

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