Complete assignment of the non-exchangeable proton nmr resonances of [d-(GGAATTCC)]2 using two-dimensional nuclear overhauser effect spectra

Abstract

Proton two-dimensional nuclear Overhauser effect (2D NOE) spectra have been obtained for [d-(GGAATTCC)]2 at a series of mixing times. By performing the experiment at 500 MHz and obtaining pure absorption phase spectra, sufficient resolution was achieved to be able to assign all seventy of the non-exchangeable proton resonances in the self-complementary octamer duplex, including the H3' resonances which overlap with the solvent peak.