Persistence of abnormal melanocytes in immunosuppressed chickens of the autoimmune "DAM" line

Abstract

The delayed amelanotic (DAM) line of chickens (Gallus gallus) is characterized by the postnatal elimination of melanocytes from regenerating feathers and from the choroid. The process of elimination is accompanied by a massive infiltration of mononuclear leukocytes (MNL) into the supporting connective tissues. When surgically bursectomized at day of hatching, chickens from this lineage develop significantly less amelanosis of the feathers. We report here a histological analysis of regenerating feathers and choroids from bursectomized birds that maintained their plumage pigmentation. In the feathers we observed the presence of morphologically abnormal melanocytes in the absence of MNL infiltration. Choroids also contained abnormal melanocytes without MNL infiltration; however, we observed a few cases of amelanotic choroids with a few MNL. These findings indicate that melanocytes of pigmented birds are morphologically abnormal even in the absence of a bursa and in the absence of leukocytic infiltrates into regenerating feathers and possibly into the choroids. We conclude from these findings that the amelanosis in unbursectomized DAM birds is due to the response of the immune system to an abnormality in the melanocytes which, by itself, does not lead to depigmentation.