Protective efficacies of live attenuated and formaldehyde-inactivated Venezuelan equine encephalitis virus vaccines against aerosol challenge in hamsters

Abstract

Although two investigational vaccines are used to immunize humans against Venezuelan equine encephalomyelitis virus, neither had previously been tested for protective efficacy against aerosol exposure. Live attenuated vaccine (TC-83) protected all hamsters challenged by either aerosol or subcutaneous routes with 4.7 to 5.2 log10 PFU of virulent Venezuelan equine encephalomyelitis virus. Formaldehyde-inactivated vaccine (C-84) failed to protect against aerosol challenge but did protect against subcutaneous challenge. Protection elicited by TC-83 vaccine did not depend solely on serum-neutralizing antibody. These studies suggest that TC-83 vaccine is preferable to C-84 vaccine for protecting laboratory workers at risk to aerosol exposure.