Human rhodopsin measurement using a T.V.-based imaging fundus reflectometer

Abstract

An imaging fundus reflectometer for in vivo mapping of rhodopsin levels is described. The instrument is based on a high-sensitivity television camera attached to a Zeiss fundus camera, which enables areas of retina of angular subtense 25 degrees to be examined at a resolution of about 1 degree. Digital processing techniques are used to average the video signal spatially and temporally and to analyse the spatial information. Measurements with an artificial eye indicate that performance is comparable to that of photomultiplier-based systems. Rhodopsin levels and regeneration data for a normal human subject are presented; these are consistent with published values. The map of visual pigment levels derived from these normal data is contrasted with that for a subject with a patchy retinal dysfunction (autosomal dominant retinitis pigmentosa).