Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Mar 15;218(3):983-5.
doi: 10.1042/bj2180983.

Copper-phenanthroline-induced site-specific oxygen-radical damage to DNA. Detection of loosely bound trace copper in biological fluids

J M Gutteridge

Copper-phenanthroline-induced site-specific oxygen-radical damage to DNA. Detection of loosely bound trace copper in biological fluids

J M Gutteridge. Biochem J. .

Abstract

Copper(II) ions, in the presence of 1,10-phenanthroline, O2 and a reducing agent, degrade DNA with the release of thiobarbituric-acid-reactive material. This reaction, dependent on the formation of oxygen radicals, was made the basis of a sensitive and specific assay for loosely bound copper in body fluids. When applied to certain extracellular fluids, trace amounts of copper could be detected in the lower micromolar range.

PubMed Disclaimer

References

    1. Biochem Med. 1978 Feb;19(1):127-32 - PubMed
    1. Ann Rheum Dis. 1978 Feb;37(1):67-70 - PubMed
    1. J Biol Chem. 1979 Dec 25;254(24):12269-72 - PubMed
    1. Biochem Biophys Res Commun. 1980 Mar 13;93(1):264-70 - PubMed
    1. Biochim Biophys Acta. 1983 Aug 23;759(1-2):38-41 - PubMed