[Isolation and properties of neurospecific proteins 14-3-2, 14-3-3 and 10-40-4]

Abstract

A procedure for isolation of three neurospecific proteins 14-3-2, 14-3-3 and 10-40-4 from human brain is proposed. This procedure includes successive ammonium sulphate precipitation, ion-exchange chromatography on DEAE-cellulose, gel filtration through Sephadex G-150 and ion-exchange chromatography on DEAE-Sephadex A-50. Specific antisera raised against these antigens were used to prove their neurospecificity and to study their species-specificity. Some physico-chemical properties of the isolated proteins were investigated. The 14-3-2 protein--neurospecific enolase, appeared to be much more stable at high temperatures than the non-neuronal enzyme isoform.