Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Apr;138(1):141-3.
doi: 10.1016/0003-2697(84)90782-6.

A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

D Wessel, U I Flügge

A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

D Wessel et al. Anal Biochem. 1984 Apr.

Abstract

A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed. The effectiveness of this method is not affected by the presence of detergents, lipids, salt, buffers, and beta-mercaptoethanol.

PubMed Disclaimer

Publication types

LinkOut - more resources