Bile acid deconjugation and attachment of chicken gut bacteria: their possible role in growth depression

Abstract

Bacteria isolated from the chicken gut were tested for their ability to deconjugate bile acids and attach to chicken epithelial cells (crop squamous cells and duodenal brush borders). Clostridium perfringens, streptococci and some of the bifidobacteria and lactobacilli were able to deconjugate all 4 substrates whereas the bacteroides deconjugated only the taurine conjugates and the coliforms were completely inactive. None of the strains of Escherichia coli or streptococci attached to squamous cells, but the anaerogenic coliform, the strain of Klebsiella aerogenes and the lactobacilli did show attachment. Attachment to brush borders was obtained with the anaerogenic coliforms, K. aerogenes, 2 out of 5 of the lactobacilli, and 4 out of 9 of the streptococci, but none of the strains of E. coli.