Application of sodium stannite in the preparation of instant [99mTc]HIDA kit

Abstract

A rapid method for the production of freeze-dried kit containing 20 mg HIDA and 0.4 mg SnCl2 X 2H2O at pH 5.5-6.0 was developed using the stannite procedure. The Sn-HIDA ligand has been prepared successfully by dissolving HIDA in stannite solution without further pH adjustment. A gelchromatography column scanning (GCS) technique has been applied to study the chemical state of technetium in the preparation. The shelf-life of the freeze-dried powder of Sn-HIDA was found stable for at least 6 months as assessed by the GCS-technique. The hepatobiliary agent has a rapid blood clearance and is excreted predominantly by the hepatobiliary system. The biodistribution study in mice showed that more than 30% of the injected dose has been accumulated in liver at 5 min postinjection and at 30 min most of the injected activity has been passed to the intestine via the biliary system.