Polynucleotides. LII. Synthesis and properties of poly(2'-deoxy-2'-fluoroadenylic acid)
- PMID: 673838
- PMCID: PMC342131
- DOI: 10.1093/nar/5.6.1877
Polynucleotides. LII. Synthesis and properties of poly(2'-deoxy-2'-fluoroadenylic acid)
Abstract
2'-Deoxy-2'-fluoroadenosine was chemically transformed to its 5'-diphosphate and polymerized with polynucleotide phosphorylase to give poly(2'-deoxy-2'-fluoroadenylic acid) [poly(Af)]. Polymerization proceeded smoothly as in the case of poly(A) and the yield of the polymerization was 55%. The UV absorption spectra of poly(Af) closely resembled those of poly(A) and the hypochromicity was 32% at pH 7.0. The CD profile at 25 degrees and neutrality showed similar pattern to that of other poly(2'-deoxy-2'-halogenoadenylic acids) with somewhat larger [theta] values both in the positive and negative maxima. Acid titration of poly(Af) showed a transition point at pH 5.2 and the Tm of the acid form was 37 degrees which was significantly lower than that of poly(A), but similar to that of poly(2'-azido-2'-deoxyadenylic acid). Poly(Af) formed 1:1 and 1:2 complexes with poly-(U) having Tm of 49 degrees and 62 degrees at 0.04M and 0.15M Na(+) concentration, respectively. Poly(Af) also formed a 1:2 complex with poly(I) and its Tm was 36 degrees at 0.05M Na(+) concentration. These data showed that poly(Af) has rather similar properties to those of poly(A), but not to poly(dA).
Similar articles
-
Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid.Nucleic Acids Res. 1976 Aug;3(8):2089-99. doi: 10.1093/nar/3.8.2089. Nucleic Acids Res. 1976. PMID: 9620 Free PMC article.
-
Polynucleotides. XLV Synthesis and properties of poly(2'-azido-2'-deoxyinosinic acid).Nucleic Acids Res. 1977 Aug;4(8):2629-39. doi: 10.1093/nar/4.8.2629. Nucleic Acids Res. 1977. PMID: 909787 Free PMC article.
-
Polynucleotides. XLVI. 1 Synthesis and properties of poly (2'-amino-2'-deoxyadenylic acid).Nucleic Acids Res. 1977 Apr;4(4):989-1000. doi: 10.1093/nar/4.4.989. Nucleic Acids Res. 1977. PMID: 17102 Free PMC article.
-
Polynucleotides. L. Synthesis and properties of poly (2'-chloro-2'-deoxyadenylic acid) and poly (2'-bromo-2'-deoxyadenylic acid).Nucleic Acids Res. 1977 Dec;4(12):4249-60. doi: 10.1093/nar/4.12.4249. Nucleic Acids Res. 1977. PMID: 341084 Free PMC article.
-
Polynucleotides. LVI. Synthesis and properties of poly(2-deoxy-2'-fluoroinosinic acid).Nucleic Acids Res. 1978 Sep;5(9):3315-24. doi: 10.1093/nar/5.9.3315. Nucleic Acids Res. 1978. PMID: 704358 Free PMC article.
Cited by
-
Structural requirements of polynucleotides for the activation of (2' - 5')An polymerase and protein kinase.Nucleic Acids Res. 1981 Oct 10;9(19):4939-50. doi: 10.1093/nar/9.19.4939. Nucleic Acids Res. 1981. PMID: 6273790 Free PMC article.
-
Polynucleotides. LVII. Synthesis and properties of poly (2'-chloro-2'-deoxyinosinic acid).Nucleic Acids Res. 1979 Jun 11;6(7):2627-36. doi: 10.1093/nar/6.7.2627. Nucleic Acids Res. 1979. PMID: 461198 Free PMC article.
-
2'-Fluoro modified nucleic acids: polymerase-directed synthesis, properties and stability to analysis by matrix-assisted laser desorption/ionization mass spectrometry.Nucleic Acids Res. 1997 Nov 15;25(22):4581-8. doi: 10.1093/nar/25.22.4581. Nucleic Acids Res. 1997. PMID: 9358169 Free PMC article.
-
Nucleoside conformation is determined by the electronegativity of the sugar substituent.Nucleic Acids Res. 1980 Mar 25;8(6):1421-33. doi: 10.1093/nar/8.6.1421. Nucleic Acids Res. 1980. PMID: 7433125 Free PMC article.
-
Translation of 2'-modified mRNA in vitro and in vivo.Nucleic Acids Res. 1994 Nov 25;22(23):4963-8. doi: 10.1093/nar/22.23.4963. Nucleic Acids Res. 1994. PMID: 7800487 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
