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. 1978 Jun;5(6):2033-8.
doi: 10.1093/nar/5.6.2033.

Assay of DNA-RNA hybrids by S1 nuclease digestion and adsorption to DEAE-cellulose filters

I H Maxwell, J Van Ness, W E Hahn
Free PMC article

Assay of DNA-RNA hybrids by S1 nuclease digestion and adsorption to DEAE-cellulose filters

I H Maxwell et al. Nucleic Acids Res. 1978 Jun.
Free PMC article

Abstract

A fast and accurate assay procedure for DNA-RNA hybrids is described in which exhaustive digestion of unhybridized DNA with S1 nuclease is followed by binding of hybrids to filter discs of DEAE-cellulose. The digested DNA can be efficiently washed from the filters so that background levels of 0.1-0.2% of input tracer DNA can be achieved, in contrast to the much higher (approximately 1-5%) backgrounds obtained using TCA precipitation procedures. Short duplexes, as small as 36 nucleotides in length, which are inefficiently bound to hydroxyapatite, are quantitatively bound to the DEAE-cellulose filters.

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References

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