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. 1978 Jun;5(6):2153-67.
doi: 10.1093/nar/5.6.2153.

DNA-methylase from regenerating rat liver: purification and characterisation

D Simon, F Grunert, U von Acken, H P Döring, H Kröger
Free PMC article

DNA-methylase from regenerating rat liver: purification and characterisation

D Simon et al. Nucleic Acids Res. 1978 Jun.
Free PMC article

Abstract

DNA methylase has been purified 660-fold from nuclei from regenerating rat liver. The enzyme is able to methylate single stranded (ss) and double stranded (ds) DNA, the only reaction product being 5-methylcytosine. Previously unmethylated double stranded DNA from prokaryotes (M.luteus) as well as from eukaryotes (Ascaris suis) can serve as substrates. The synthetic copolymers (dG-dC)n . (dC-dG)n and (dG,dC)n are also methylated. While SV40 DNA is almost not methylated, PM2 DNA is a good substrate even in the supercoiled form. The enzyme methylates 1 in 17 bases in heterologous M.luteus DNA, but only 1 in 590 in homologous rat liver DNA. The high methylation level of M.luteus DNA, an analysis of the methylated pyrimidine isostichs and a preliminary dinucleotide analysis suggest that all the CpGs in a DNA can be methylated.

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