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. 1978 Jul;5(7):2373-80.
doi: 10.1093/nar/5.7.2373.

A general method for the purification of restriction enzymes

P J Greene, H L Heyneker, F Bolivar, R L Rodriguez, M C Betlach, A A Covarrubias, K Backman, D J Russel, R Tait, H W Boyer
Free PMC article

A general method for the purification of restriction enzymes

P J Greene et al. Nucleic Acids Res. 1978 Jul.
Free PMC article

Abstract

An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.

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References

    1. Gene. 1977;2(2):95-113 - PubMed
    1. CRC Crit Rev Biochem. 1976 Nov;4(2):123-64 - PubMed
    1. Annu Rev Biochem. 1975;44:273-93 - PubMed

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