doi: 10.1093/nar/5.7.2373.
A general method for the purification of restriction enzymes
- PMID: 673857
- PMCID: PMC342170
- DOI: 10.1093/nar/5.7.2373
Item in Clipboard
A general method for the purification of restriction enzymes
Nucleic Acids Res.
1978 Jul.
Free PMC article
Abstract
An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.
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