[Effect of noradrenaline on the growth of tissues cultures of rat cerebral cortex]

Abstract

Morpho-functional development of the cerebral cortex of newborn rats and the influence of noradrenaline (NA) on this process was investigated under the conditions of long-term (up to 41 days) organotypical tissue cultivation. Systematic adding of NA to the nutrient medium in the process of explants cultivation was shown to disturb its structural organization, to depress proliferation of the glia, synaptogenesis and axon myelinization. Decrease of number of spontaneously active neurones by 30% and predominance of neurones with periodical discharges (bursts or groups) was observed in all periods of cultivating in NA presence. Development of neurones in the NA containing medium changed their sensitivity to NA which became close to that in situ.